glass beads 0.1mm 11079101 (BioSpec)

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BioSpec glass beads 0.1mm 11079101
Glass Beads 0.1mm 11079101, supplied by BioSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glass+beads+0%2E1mm/glass+beads+0+1mm+11079101/pmc12038827-371-17-19
Average 90 stars, based on 1 article reviews

glass beads 0.1mm 11079101 - by Bioz Stars, 2026-11

90/100 stars

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Labeling:

Article Title: Essential role of membrane vesicles for biological activity of the bacteriocin micrococcin P1.
Article Snippet: .. Seven microgram of labeled MVs were applied to 1 × 108 S. aureus or A. baumannii cells and fluorescence was measured for 30 min using a fluorescent plate reader (Synergy H1) with an excitation wavelength of 560 nm and an emission wavelength of 590 nm. . MV lysis and proteinase K treatment Hundred microlitre aliquots of MV solutions (∼20 μg MVs per aliquot) were pipetted into 2-ml tubes, mixed well with 60 mg glass beads (0.1mm, Biospec) and lysed using a FastPrep-96 Sample Preparation Instrument (14,000 rpm/min for 1min). .. Samples were then centrifuged at 50,000 × g at 4◦C for 1 min, transferred to a new 1.5-ml tube, and incubated with 20 μg/ml proteinase K (PK, MP Biomedicals) at 37◦C for 1 h. Control samples did not undergo lysis and/or PK treatment, but were mixed with beads to control for the volume loss associated with the procedure and for possible adherence to beads.

Fluorescence:

Lysis:

Article Title: Formation of a membraneless compartment regulates bacterial virulence.
Article Snippet: .. Bacteria were then suspended in 800 μl of lysis/washing buffer containing 50mM sodium phosphate, 300mM NaCl, 1:50 complete protease inhibitor-EDTA free (Roche), loaded onto glass beads 0.1mm (Biospec, Cat. 11079101) and lysed using the mixer mill (Retch, model no. MM400). .. The crude bacterial lysate was cleared by centrifugation and further incubated with 20 μl of anti-FLAGmagnetic beads (M8823, sigma) for 1 h at 4 °C.

Article Title: Probiotic treatment induces changes in intestinal microbiota but does not alter SCFA levels in peritoneal dialysis patients—a randomized, placebo-controlled trial
Article Snippet: .. The fecal sample was suspended in 790μl of sterile lysis buffer (4M guanidine thiocyanate; 10% N-lauroyl sarcosine; 5% N-lauroyl sarcosine-0.1 M phosphate buffer [pH 8.0]) in 2 ml screw-cap tube containing 1g glass beads (0.1mm BioSpec Products, Inc., USA). ..

Article Title: A Comparative Study of the Gut Microbiota Associated With Immunoglobulin a Nephropathy and Membranous Nephropathy.
Article Snippet: .. The fecal sample was added to a 2ml screwcap vial containing 1 g glass beads (0.1mm BioSpec Products, Inc., USA) and was suspended in 790 μl sterile lysis buffer (4M guanidine thiocyanate; 10% Nlauroylsarcosine; 5%N-lauroyl sarcosine-0.1M phosphate buffer, pH 8.0). .. The samples were subjected to bead beating for 10min at maximum speed prior to incubation at 70◦C for 1 h. Microbial DNA was extracted using the E.Z.N.A R©Stool DNA kit (Omega Bio-tek, Inc., GA,USA).

Article Title: Effects of a high-phosphorus diet on the gut microbiota in CKD rats
Article Snippet: A.R Stool DNA Kit (Omega Bio-tek, Inc., GA). .. In summary, 0.2 g of feces were thawed and added 790mL lysis buffer containing 4M guanidine thiocyanate, 10% N-lauroyl sarcosine, 5% N-lauroyl sarcosine-0.1 M phosphate Buffer [pH 8.0], and 1 g glass beads (0.1mm, Biospec Products, Inc., USA). ..

Sample Prep:

Bacteria:

Sterility:

Article Title: Microbial community and abiotic effects on aquatic bacterial communities in north temperate lakes.
Article Snippet: • Freshwater microbial community structure was assessed in 60 lakes.. • Bacterial community structure is primarily deterministic, not stochastic.. • Both biotic and abiotic factors contributed to bacterial community

Article Title: Cysteine dependence of Lactobacillus iners is a potential therapeutic target for vaginal microbiota modulation
Article Snippet: Unless otherwise indicated, all growth, growth inhibition, isotopic tracing, and mock community experiments employed L. crispatus strain 233, L. iners strain F1, and G. vaginalis strain ATCC 14018. .. Swabs were thawed on ice, transferred into a solution consisting of phenol:chloroform:isoamyl alcohol (“PCI”, 25:24:1, pH 7.9, Ambion) and 20% sodium dodecyl sulfate (Fisher) in Tris-EDTA buffer with sterile 0.1mm glass beads (BioSpec), vigorously rubbed against the walls of the tube to dislodge microbial material, then incubated on ice for 5–10 minutes. ..

Filtration:

Ethanol Precipitation:

Article Title: Interpersonal Gut Microbiome Variation Drives Susceptibility and Resistance to Cholera Infection.
Article Snippet: The B. obeum luxS coding region (from genome position 33,305- 33,784) was codon-optimized for expression in E. coli, placed downstream of the PLtet-O-1 constitutive promoter sequence derived from the plasmid vector pZE21 vector, and the construct cloned into vector pMK using the GeneArt Subcloning & Express Cloning Service (ThermoFisher). .. 500 mL 0.1mm glass beads (BioSpec), 210 mL SDS %20, and 500 mL neutral phenol:chloroform:isoamyl-alcohol (24:24:1, Fisher Scientific) were added to each sample, and samples were lysed by bead-beating followed by ethanol precipitation (Hsiao et al., 2014). .. The V4 variable region of bacterial 16S ribosomal RNA genes was amplified in 25 mL total volume reactions comprising 1 mL of extracted DNA as template, 10 mL Platinum Hot Start PCRMaster Mix (ThermoFisher), 13 mL PCR-grade water and 0.5 mL of forward and reverse primers (10 mM).

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